Measured Retention Times for Preloaded Data Sets

There are a total of 10 sets of experimental retention times preloaded into the software. They were acquired from 5 different experimental conditions run on two different HPLC instruments. The experimental retention times of the gradient calibration solutes can be automatically entered by clicking on the "Use Preloaded Values" button and selecting a dataset. The experimental retention times of the other database solutes are shown below. You can use these retention times to check the accuracy of retention times predicted from back-calculated gradient and flow rate profiles.

aN.D. means not determined.

Primary Instrument: Thermo Scientific Inc. Accela UHPLC Pump

Condition 1: 10% B to 100% B in 5 min at 200 μL/min

Condition 2: 10% B to 100% B in 20 min at 100 μL/min

Condition 3: 10% B to 100% B in 20 min at 200 μL/min

Condition 4: 10% B to 100% B in 20 min at 400 μL/min

Condition 5: 10% B to 100% B in 80 min at 200 μL/min

Secondary Instrument: Waters Corporation Acquity UPLC Pump

Condition 1: 10% B to 100% B in 5 min at 200 μL/min

Condition 2: 10% B to 100% B in 20 min at 100 μL/min

Condition 3: 10% B to 100% B in 20 min at 200 μL/min

Condition 4: 10% B to 100% B in 20 min at 400 μL/min

Condition 5: 10% B to 100% B in 80 min at 200 μL/min